IFI-B · Naringin Reduction Series

Specimen SS-F-BTR-549 // ZERO-BITTER · IFI-B

Naringin Reduc · T4 Protoplast-A

Citrus sinensis

Orange

Tier IVNaringin Reduction Series

Target Trait

70%

% reduction

Naringin reduction

Technical Specifications

SKU

SS-F-BTR-549

Genomic Edits

7

GC Content

43.4%

Construct Length

3,917 bp

Promoter System

35S CaMV / RbcS

Expression System

PEG-mediated protoplast transfection

Host Strain

Isolated leaf mesophyll protoplasts

Receptor Target

TAS2R38 (suppression)

IFI Score

70

Pathway Edits

  • RhaT rhamnosyltransferase RNAi prevents final rhamnosylation of naringenin-7-glucoside to bitter naringin
  • GT1 glucosyltransferase alternative isoform redirects naringenin to non-bitter glycoside conjugates
  • CHS chalcone synthase RNAi reduces total naringenin precursor pool available for glycosylation
  • CHI chalcone isomerase modification channels chalcone flux away from naringenin accumulation

Key Interventions

RhaT rhamnosyltransferase RNAiGT1 isoform redirectionCHS chalcone suppression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
70
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV Citrus sinensis lines with validated naringin reduction for fresh juice processing, citrus-flavored beverages, and functional food applications where grapefruit-like bitterness would otherwise activate TAS2R38 to objectionable levels.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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