Specimen SS-F-BTR-543 // ZERO-BITTER · IFI-B
Naringin Reduc · T3 Cell-Free-A
Citrus sinensis
Orange
Target Trait
60%
% reduction
Naringin reduction
Technical Specifications
SKU
SS-F-BTR-543
Genomic Edits
6
GC Content
43.8%
Construct Length
3,604 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
60
Pathway Edits
- RhaT rhamnosyltransferase RNAi prevents final rhamnosylation of naringenin-7-glucoside to bitter naringin
- GT1 glucosyltransferase alternative isoform redirects naringenin to non-bitter glycoside conjugates
- CHS chalcone synthase RNAi reduces total naringenin precursor pool available for glycosylation
- CHI chalcone isomerase modification channels chalcone flux away from naringenin accumulation
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier III Citrus sinensis lines with validated naringin reduction for fresh juice processing, citrus-flavored beverages, and functional food applications where grapefruit-like bitterness would otherwise activate TAS2R38 to objectionable levels.
License
$1499 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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