Specimen SS-F-BTR-516 // ZERO-BITTER · IFI-B
Catechin Bitt · T5 Biolistic-B
Camellia sinensis
Tea
Target Trait
65%
% reduction
EGCG bitterness reduction
Technical Specifications
SKU
SS-F-BTR-516
Genomic Edits
7
GC Content
48.7%
Construct Length
3,987 bp
Promoter System
UBI-1 / 35S CaMV
Expression System
Biolistic particle bombardment
Host Strain
Gold particle 0.6 µm
Receptor Target
TAS2R38 (suppression)
IFI Score
65
Pathway Edits
- ANR anthocyanidin reductase RNAi shifts flavan-3-ol flux away from EGCG accumulation
- LAR leucoanthocyanidin reductase RNAi reduces catechin/epicatechin precursor pool for galloylation
- F3H flavanone 3-hydroxylase modification limits dihydroflavonol substrate for catechin entry
- DFR dihydroflavonol 4-reductase engineering reduces anthocyanidin branch competition pressure
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier V Camellia sinensis lines with reduced EGCG content for applications requiring green tea flavor notes with diminished astringency and bitterness, including white tea formulations, cold-brew concentrates, and sensitive palate product lines.
License
$10049 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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