IFI-B · Catechin Bitterness Series

Specimen SS-F-BTR-514 // ZERO-BITTER · IFI-B

Catechin Bitt · T5 Agrobacterium-B

Camellia sinensis

Tea

Tier VCatechin Bitterness Series

Target Trait

65%

% reduction

EGCG bitterness reduction

Technical Specifications

SKU

SS-F-BTR-514

Genomic Edits

7

GC Content

47.5%

Construct Length

3,953 bp

Promoter System

35S CaMV / mas2

Expression System

T-DNA binary vector (pCAMBIA)

Host Strain

Agrobacterium tumefaciens EHA105

Receptor Target

TAS2R38 (suppression)

IFI Score

65

Pathway Edits

  • ANR anthocyanidin reductase RNAi shifts flavan-3-ol flux away from EGCG accumulation
  • LAR leucoanthocyanidin reductase RNAi reduces catechin/epicatechin precursor pool for galloylation
  • F3H flavanone 3-hydroxylase modification limits dihydroflavonol substrate for catechin entry
  • DFR dihydroflavonol 4-reductase engineering reduces anthocyanidin branch competition pressure

Key Interventions

ANR RNAi knockdownLAR reductase silencingF3H flavanone modification

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
65
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier V Camellia sinensis lines with reduced EGCG content for applications requiring green tea flavor notes with diminished astringency and bitterness, including white tea formulations, cold-brew concentrates, and sensitive palate product lines.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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