IFI-B · Catechin Bitterness Series

Specimen SS-F-BTR-512 // ZERO-BITTER · IFI-B

Catechin Bitt · T4 Cell-Free-B

Camellia sinensis

Tea

Tier IVCatechin Bitterness Series

Target Trait

56%

% reduction

EGCG bitterness reduction

Technical Specifications

SKU

SS-F-BTR-512

Genomic Edits

6

GC Content

46.2%

Construct Length

3,508 bp

Promoter System

T7 / SP6 RNA polymerase

Expression System

Cell-free in vitro transcription/translation

Host Strain

In vitro wheat germ extract

Receptor Target

TAS2R38 (suppression)

IFI Score

56

Pathway Edits

  • ANR anthocyanidin reductase RNAi shifts flavan-3-ol flux away from EGCG accumulation
  • LAR leucoanthocyanidin reductase RNAi reduces catechin/epicatechin precursor pool for galloylation
  • F3H flavanone 3-hydroxylase modification limits dihydroflavonol substrate for catechin entry
  • DFR dihydroflavonol 4-reductase engineering reduces anthocyanidin branch competition pressure

Key Interventions

ANR RNAi knockdownLAR reductase silencingF3H flavanone modification

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
56
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV Camellia sinensis lines with reduced EGCG content for applications requiring green tea flavor notes with diminished astringency and bitterness, including white tea formulations, cold-brew concentrates, and sensitive palate product lines.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Zero-Bitter · IFI-BAll Modalities