Specimen SS-F-BTR-509 // ZERO-BITTER · IFI-B
Catechin Bitt · T4 Protoplast-A
Camellia sinensis
Tea
Target Trait
56%
% reduction
EGCG bitterness reduction
Technical Specifications
SKU
SS-F-BTR-509
Genomic Edits
6
GC Content
43.4%
Construct Length
3,657 bp
Promoter System
35S CaMV / RbcS
Expression System
PEG-mediated protoplast transfection
Host Strain
Isolated leaf mesophyll protoplasts
Receptor Target
TAS2R38 (suppression)
IFI Score
56
Pathway Edits
- ANR anthocyanidin reductase RNAi shifts flavan-3-ol flux away from EGCG accumulation
- LAR leucoanthocyanidin reductase RNAi reduces catechin/epicatechin precursor pool for galloylation
- F3H flavanone 3-hydroxylase modification limits dihydroflavonol substrate for catechin entry
- DFR dihydroflavonol 4-reductase engineering reduces anthocyanidin branch competition pressure
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier IV Camellia sinensis lines with reduced EGCG content for applications requiring green tea flavor notes with diminished astringency and bitterness, including white tea formulations, cold-brew concentrates, and sensitive palate product lines.
License
$3999 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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