Specimen SS-F-BTR-487 // ZERO-BITTER · IFI-B
Catechin Bitt · T1 Cell-Free-A
Camellia sinensis
Tea
Target Trait
30%
% reduction
EGCG bitterness reduction
Technical Specifications
SKU
SS-F-BTR-487
Genomic Edits
3
GC Content
44.7%
Construct Length
2,450 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
30
Pathway Edits
- ANR anthocyanidin reductase RNAi shifts flavan-3-ol flux away from EGCG accumulation
- LAR leucoanthocyanidin reductase RNAi reduces catechin/epicatechin precursor pool for galloylation
- F3H flavanone 3-hydroxylase modification limits dihydroflavonol substrate for catechin entry
- DFR dihydroflavonol 4-reductase engineering reduces anthocyanidin branch competition pressure
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier I Camellia sinensis lines with reduced EGCG content for applications requiring green tea flavor notes with diminished astringency and bitterness, including white tea formulations, cold-brew concentrates, and sensitive palate product lines.
License
$299 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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