Specimen SS-F-BTR-486 // ZERO-BITTER · IFI-B
Catechin Bitt · T1 Protoplast-B
Camellia sinensis
Tea
Target Trait
30%
% reduction
EGCG bitterness reduction
Technical Specifications
SKU
SS-F-BTR-486
Genomic Edits
3
GC Content
48.5%
Construct Length
2,433 bp
Promoter System
35S CaMV / RbcS
Expression System
PEG-mediated protoplast transfection
Host Strain
Isolated leaf mesophyll protoplasts
Receptor Target
TAS2R38 (suppression)
IFI Score
30
Pathway Edits
- ANR anthocyanidin reductase RNAi shifts flavan-3-ol flux away from EGCG accumulation
- LAR leucoanthocyanidin reductase RNAi reduces catechin/epicatechin precursor pool for galloylation
- F3H flavanone 3-hydroxylase modification limits dihydroflavonol substrate for catechin entry
- DFR dihydroflavonol 4-reductase engineering reduces anthocyanidin branch competition pressure
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier I Camellia sinensis lines with reduced EGCG content for applications requiring green tea flavor notes with diminished astringency and bitterness, including white tea formulations, cold-brew concentrates, and sensitive palate product lines.
License
$349 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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