Specimen SS-F-BTR-472 // ZERO-BITTER · IFI-B
Caffeine Reduc · T4 Cell-Free-B
Camellia sinensis
Tea
Target Trait
75%
% reduction
Caffeine (methylxanthine) reduction
Technical Specifications
SKU
SS-F-BTR-472
Genomic Edits
8
GC Content
46.2%
Construct Length
4,388 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
75
Pathway Edits
- DXMT 3,7-dimethylxanthine N-methyltransferase RNAi blocks final methylation converting theobromine to caffeine
- TCSAMT 7-methylxanthine N-methyltransferase RNAi reduces theobromine-to-caffeine conversion efficiency
- CS caffeine synthase partial antisense reduces overall N-methylation capacity in young leaves
- XDH xanthine dehydrogenase engineering redirects xanthine oxidation away from the methylation pathway
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier IV Camellia sinensis lines for naturally low-caffeine tea production, decaffeinated specialty tea formulations, and pediatric or sensitive-population beverage applications where methylxanthine content must be strictly controlled without solvent-based decaffeination.
License
$4049 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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