Specimen SS-F-BTR-462 // ZERO-BITTER · IFI-B
Caffeine Reduc · T3 Protoplast-B
Camellia sinensis
Tea
Target Trait
65%
% reduction
Caffeine (methylxanthine) reduction
Technical Specifications
SKU
SS-F-BTR-462
Genomic Edits
7
GC Content
47.6%
Construct Length
4,007 bp
Promoter System
35S CaMV / RbcS
Expression System
PEG-mediated protoplast transfection
Host Strain
Isolated leaf mesophyll protoplasts
Receptor Target
TAS2R38 (suppression)
IFI Score
65
Pathway Edits
- DXMT 3,7-dimethylxanthine N-methyltransferase RNAi blocks final methylation converting theobromine to caffeine
- TCSAMT 7-methylxanthine N-methyltransferase RNAi reduces theobromine-to-caffeine conversion efficiency
- CS caffeine synthase partial antisense reduces overall N-methylation capacity in young leaves
- XDH xanthine dehydrogenase engineering redirects xanthine oxidation away from the methylation pathway
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier III Camellia sinensis lines for naturally low-caffeine tea production, decaffeinated specialty tea formulations, and pediatric or sensitive-population beverage applications where methylxanthine content must be strictly controlled without solvent-based decaffeination.
License
$1549 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
Already purchased?