Specimen SS-F-BTR-460 // ZERO-BITTER · IFI-B
Caffeine Reduc · T3 Biolistic-B
Camellia sinensis
Tea
Target Trait
65%
% reduction
Caffeine (methylxanthine) reduction
Technical Specifications
SKU
SS-F-BTR-460
Genomic Edits
7
GC Content
46.5%
Construct Length
3,973 bp
Promoter System
UBI-1 / 35S CaMV
Expression System
Biolistic particle bombardment
Host Strain
Gold particle 0.6 µm
Receptor Target
TAS2R38 (suppression)
IFI Score
65
Pathway Edits
- DXMT 3,7-dimethylxanthine N-methyltransferase RNAi blocks final methylation converting theobromine to caffeine
- TCSAMT 7-methylxanthine N-methyltransferase RNAi reduces theobromine-to-caffeine conversion efficiency
- CS caffeine synthase partial antisense reduces overall N-methylation capacity in young leaves
- XDH xanthine dehydrogenase engineering redirects xanthine oxidation away from the methylation pathway
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier III Camellia sinensis lines for naturally low-caffeine tea production, decaffeinated specialty tea formulations, and pediatric or sensitive-population beverage applications where methylxanthine content must be strictly controlled without solvent-based decaffeination.
License
$1549 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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