IFI-B · Caffeine Reduction Series

Specimen SS-F-BTR-445 // ZERO-BITTER · IFI-B

Caffeine Reduc · T1 Protoplast-A

Camellia sinensis

Tea

Tier ICaffeine Reduction Series

Target Trait

45%

% reduction

Caffeine (methylxanthine) reduction

Technical Specifications

SKU

SS-F-BTR-445

Genomic Edits

4

GC Content

43.9%

Construct Length

2,916 bp

Promoter System

35S CaMV / RbcS

Expression System

PEG-mediated protoplast transfection

Host Strain

Isolated leaf mesophyll protoplasts

Receptor Target

TAS2R38 (suppression)

IFI Score

45

Pathway Edits

  • DXMT 3,7-dimethylxanthine N-methyltransferase RNAi blocks final methylation converting theobromine to caffeine
  • TCSAMT 7-methylxanthine N-methyltransferase RNAi reduces theobromine-to-caffeine conversion efficiency
  • CS caffeine synthase partial antisense reduces overall N-methylation capacity in young leaves
  • XDH xanthine dehydrogenase engineering redirects xanthine oxidation away from the methylation pathway

Key Interventions

DXMT RNAi silencingTCSAMT N-methyltransferase suppressionCS antisense construct

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
45
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier I Camellia sinensis lines for naturally low-caffeine tea production, decaffeinated specialty tea formulations, and pediatric or sensitive-population beverage applications where methylxanthine content must be strictly controlled without solvent-based decaffeination.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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