IFI-B · Chlorogenic Acid Reduction Series

Specimen SS-F-BTR-433 // ZERO-BITTER · IFI-B

Chlorogenic Acid · T5 Agrobacterium-A

Coffea arabica

Coffee

Tier VChlorogenic Acid Reduction Series

Target Trait

80%

% reduction

Chlorogenic acid (CGA) reduction

Technical Specifications

SKU

SS-F-BTR-433

Genomic Edits

9

GC Content

45.9%

Construct Length

4,736 bp

Promoter System

35S CaMV / mas2

Expression System

T-DNA binary vector (pCAMBIA)

Host Strain

Agrobacterium tumefaciens EHA105

Receptor Target

TAS2R38 (suppression)

IFI Score

80

Pathway Edits

  • HCT hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyltransferase RNAi reduces CGA priming step
  • C3H p-coumarate 3-hydroxylase RNAi limits caffeic acid availability for CGA conjugation
  • CST chlorogenate synthase RNAi blocks final ester-bond formation step in CGA assembly
  • QT quinate/shikimate pathway throttle attenuates total CGA throughput at the branch point

Key Interventions

HCT RNAi suppressionC3H pathway blockCST chlorogenate synthase silencing

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
80
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier V Coffea arabica lines with substantially reduced CGA content for specialty coffee roasters, low-acid coffee formulations, and functional beverage applications where bitter TAS2R38 stimulation must be minimized without altering roast aroma development.

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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