Specimen SS-F-BTR-432 // ZERO-BITTER · IFI-B
Chlorogenic Acid · T4 Cell-Free-B
Coffea arabica
Coffee
Target Trait
70%
% reduction
Chlorogenic acid (CGA) reduction
Technical Specifications
SKU
SS-F-BTR-432
Genomic Edits
8
GC Content
46.2%
Construct Length
4,308 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
70
Pathway Edits
- HCT hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyltransferase RNAi reduces CGA priming step
- C3H p-coumarate 3-hydroxylase RNAi limits caffeic acid availability for CGA conjugation
- CST chlorogenate synthase RNAi blocks final ester-bond formation step in CGA assembly
- QT quinate/shikimate pathway throttle attenuates total CGA throughput at the branch point
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier IV Coffea arabica lines with substantially reduced CGA content for specialty coffee roasters, low-acid coffee formulations, and functional beverage applications where bitter TAS2R38 stimulation must be minimized without altering roast aroma development.
License
$4049 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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