Specimen SS-F-BTR-410 // ZERO-BITTER · IFI-B
Chlorogenic Acid · T2 Agrobacterium-B
Coffea arabica
Coffee
Target Trait
50%
% reduction
Chlorogenic acid (CGA) reduction
Technical Specifications
SKU
SS-F-BTR-410
Genomic Edits
5
GC Content
47.8%
Construct Length
3,132 bp
Promoter System
35S CaMV / mas2
Expression System
T-DNA binary vector (pCAMBIA)
Host Strain
Agrobacterium tumefaciens EHA105
Receptor Target
TAS2R38 (suppression)
IFI Score
50
Pathway Edits
- HCT hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyltransferase RNAi reduces CGA priming step
- C3H p-coumarate 3-hydroxylase RNAi limits caffeic acid availability for CGA conjugation
- CST chlorogenate synthase RNAi blocks final ester-bond formation step in CGA assembly
- QT quinate/shikimate pathway throttle attenuates total CGA throughput at the branch point
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier II Coffea arabica lines with substantially reduced CGA content for specialty coffee roasters, low-acid coffee formulations, and functional beverage applications where bitter TAS2R38 stimulation must be minimized without altering roast aroma development.
License
$749 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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