Specimen SS-F-BTR-408 // ZERO-BITTER · IFI-B
Chlorogenic Acid · T1 Cell-Free-B
Coffea arabica
Coffee
Target Trait
40%
% reduction
Chlorogenic acid (CGA) reduction
Technical Specifications
SKU
SS-F-BTR-408
Genomic Edits
4
GC Content
46.0%
Construct Length
2,887 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
40
Pathway Edits
- HCT hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyltransferase RNAi reduces CGA priming step
- C3H p-coumarate 3-hydroxylase RNAi limits caffeic acid availability for CGA conjugation
- CST chlorogenate synthase RNAi blocks final ester-bond formation step in CGA assembly
- QT quinate/shikimate pathway throttle attenuates total CGA throughput at the branch point
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier I Coffea arabica lines with substantially reduced CGA content for specialty coffee roasters, low-acid coffee formulations, and functional beverage applications where bitter TAS2R38 stimulation must be minimized without altering roast aroma development.
License
$349 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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