Specimen SS-F-BTR-400 // ZERO-BITTER · IFI-B
Humulone α-Acid · T5 Cell-Free-B
Humulus lupulus
Hops
Target Trait
70%
% reduction
Humulone (α-acid) reduction
Technical Specifications
SKU
SS-F-BTR-400
Genomic Edits
8
GC Content
47.4%
Construct Length
4,395 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
70
Pathway Edits
- HPT1 prenyltransferase RNAi reduces isopentyl diphosphate prenylation onto phlorisovalerophenone
- OMT1 O-methyltransferase RNAi disrupts the methylphloracetophenone branch feeding humulone
- CHS_H1 chalcone synthase attenuation reduces phlorisovalerophenone substrate pool for HPT1
- 4CL 4-coumarate:CoA ligase modification limits feruloyl-CoA availability for α-acid priming
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier V Humulus lupulus lines with reduced α-acid load for craft brewing applications, low-bitter hop extracts, and non-alcoholic beverage formulations where controlled bitterness is specified in the product brief.
License
$10049 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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