Specimen SS-F-BTR-389 // ZERO-BITTER · IFI-B
Humulone α-Acid · T4 Protoplast-A
Humulus lupulus
Hops
Target Trait
61%
% reduction
Humulone (α-acid) reduction
Technical Specifications
SKU
SS-F-BTR-389
Genomic Edits
7
GC Content
43.4%
Construct Length
3,997 bp
Promoter System
35S CaMV / RbcS
Expression System
PEG-mediated protoplast transfection
Host Strain
Isolated leaf mesophyll protoplasts
Receptor Target
TAS2R38 (suppression)
IFI Score
61
Pathway Edits
- HPT1 prenyltransferase RNAi reduces isopentyl diphosphate prenylation onto phlorisovalerophenone
- OMT1 O-methyltransferase RNAi disrupts the methylphloracetophenone branch feeding humulone
- CHS_H1 chalcone synthase attenuation reduces phlorisovalerophenone substrate pool for HPT1
- 4CL 4-coumarate:CoA ligase modification limits feruloyl-CoA availability for α-acid priming
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier IV Humulus lupulus lines with reduced α-acid load for craft brewing applications, low-bitter hop extracts, and non-alcoholic beverage formulations where controlled bitterness is specified in the product brief.
License
$3999 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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