IFI-B · Humulone Alpha-Acid Reduction Series

Specimen SS-F-BTR-387 // ZERO-BITTER · IFI-B

Humulone α-Acid · T4 Biolistic-A

Humulus lupulus

Hops

Tier IVHumulone Alpha-Acid Reduction Series

Target Trait

61%

% reduction

Humulone (α-acid) reduction

Technical Specifications

SKU

SS-F-BTR-387

Genomic Edits

7

GC Content

45.6%

Construct Length

3,963 bp

Promoter System

UBI-1 / 35S CaMV

Expression System

Biolistic particle bombardment

Host Strain

Gold particle 0.6 µm

Receptor Target

TAS2R38 (suppression)

IFI Score

61

Pathway Edits

  • HPT1 prenyltransferase RNAi reduces isopentyl diphosphate prenylation onto phlorisovalerophenone
  • OMT1 O-methyltransferase RNAi disrupts the methylphloracetophenone branch feeding humulone
  • CHS_H1 chalcone synthase attenuation reduces phlorisovalerophenone substrate pool for HPT1
  • 4CL 4-coumarate:CoA ligase modification limits feruloyl-CoA availability for α-acid priming

Key Interventions

HPT1 prenylase RNAiOMT1 methyltransferase silencingCHS_H1 attenuation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
61
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV Humulus lupulus lines with reduced α-acid load for craft brewing applications, low-bitter hop extracts, and non-alcoholic beverage formulations where controlled bitterness is specified in the product brief.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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