IFI-B · Humulone Alpha-Acid Reduction Series

Specimen SS-F-BTR-375 // ZERO-BITTER · IFI-B

Humulone α-Acid · T2 Cell-Free-A

Humulus lupulus

Hops

Tier IIHumulone Alpha-Acid Reduction Series

Target Trait

44%

% reduction

Humulone (α-acid) reduction

Technical Specifications

SKU

SS-F-BTR-375

Genomic Edits

5

GC Content

45.4%

Construct Length

3,137 bp

Promoter System

T7 / SP6 RNA polymerase

Expression System

Cell-free in vitro transcription/translation

Host Strain

In vitro wheat germ extract

Receptor Target

TAS2R38 (suppression)

IFI Score

44

Pathway Edits

  • HPT1 prenyltransferase RNAi reduces isopentyl diphosphate prenylation onto phlorisovalerophenone
  • OMT1 O-methyltransferase RNAi disrupts the methylphloracetophenone branch feeding humulone
  • CHS_H1 chalcone synthase attenuation reduces phlorisovalerophenone substrate pool for HPT1
  • 4CL 4-coumarate:CoA ligase modification limits feruloyl-CoA availability for α-acid priming

Key Interventions

HPT1 prenylase RNAiOMT1 methyltransferase silencingCHS_H1 attenuation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
44
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier II Humulus lupulus lines with reduced α-acid load for craft brewing applications, low-bitter hop extracts, and non-alcoholic beverage formulations where controlled bitterness is specified in the product brief.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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