Specimen SS-F-BTR-368 // ZERO-BITTER · IFI-B
Humulone α-Acid · T1 Cell-Free-B
Humulus lupulus
Hops
Target Trait
35%
% reduction
Humulone (α-acid) reduction
Technical Specifications
SKU
SS-F-BTR-368
Genomic Edits
4
GC Content
46.0%
Construct Length
2,807 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
35
Pathway Edits
- HPT1 prenyltransferase RNAi reduces isopentyl diphosphate prenylation onto phlorisovalerophenone
- OMT1 O-methyltransferase RNAi disrupts the methylphloracetophenone branch feeding humulone
- CHS_H1 chalcone synthase attenuation reduces phlorisovalerophenone substrate pool for HPT1
- 4CL 4-coumarate:CoA ligase modification limits feruloyl-CoA availability for α-acid priming
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier I Humulus lupulus lines with reduced α-acid load for craft brewing applications, low-bitter hop extracts, and non-alcoholic beverage formulations where controlled bitterness is specified in the product brief.
License
$349 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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