Specimen SS-F-BTR-357 // ZERO-BITTER · IFI-B
Indole-GLS Mod · T5 Protoplast-A
Brassica oleracea
Broccoli/Kale
Target Trait
65%
% reduction
Indole GLS (neoglucobrassicin) reduction
Technical Specifications
SKU
SS-F-BTR-357
Genomic Edits
7
GC Content
44.4%
Construct Length
3,884 bp
Promoter System
35S CaMV / RbcS
Expression System
PEG-mediated protoplast transfection
Host Strain
Isolated leaf mesophyll protoplasts
Receptor Target
TAS2R38 (suppression)
IFI Score
65
Pathway Edits
- CYP79B2 RNAi suppresses Trp-to-indole-3-acetaldoxime conversion at the indole GLS entry step
- CYP79B3 RNAi provides redundant silencing coverage of the IAOx branch
- MYB34 transcriptional repressor overexpression down-regulates indole GLS biosynthetic network
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier V kale and broccoli lines with specifically reduced indole glucosinolate burden, designed for formulations where aliphatic GLS flavor notes are desired but neoglucobrassicin-associated bitterness is objectionable.
License
$9999 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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