Specimen SS-F-BTR-349 // ZERO-BITTER · IFI-B
Indole-GLS Mod · T4 Protoplast-A
Brassica oleracea
Broccoli/Kale
Target Trait
56%
% reduction
Indole GLS (neoglucobrassicin) reduction
Technical Specifications
SKU
SS-F-BTR-349
Genomic Edits
6
GC Content
43.4%
Construct Length
3,537 bp
Promoter System
35S CaMV / RbcS
Expression System
PEG-mediated protoplast transfection
Host Strain
Isolated leaf mesophyll protoplasts
Receptor Target
TAS2R38 (suppression)
IFI Score
56
Pathway Edits
- CYP79B2 RNAi suppresses Trp-to-indole-3-acetaldoxime conversion at the indole GLS entry step
- CYP79B3 RNAi provides redundant silencing coverage of the IAOx branch
- MYB34 transcriptional repressor overexpression down-regulates indole GLS biosynthetic network
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier IV kale and broccoli lines with specifically reduced indole glucosinolate burden, designed for formulations where aliphatic GLS flavor notes are desired but neoglucobrassicin-associated bitterness is objectionable.
License
$3999 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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