Specimen SS-F-BTR-335 // ZERO-BITTER · IFI-B
Indole-GLS Mod · T2 Cell-Free-A
Brassica oleracea
Broccoli/Kale
Target Trait
39%
% reduction
Indole GLS (neoglucobrassicin) reduction
Technical Specifications
SKU
SS-F-BTR-335
Genomic Edits
4
GC Content
45.4%
Construct Length
2,877 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
39
Pathway Edits
- CYP79B2 RNAi suppresses Trp-to-indole-3-acetaldoxime conversion at the indole GLS entry step
- CYP79B3 RNAi provides redundant silencing coverage of the IAOx branch
- MYB34 transcriptional repressor overexpression down-regulates indole GLS biosynthetic network
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier II kale and broccoli lines with specifically reduced indole glucosinolate burden, designed for formulations where aliphatic GLS flavor notes are desired but neoglucobrassicin-associated bitterness is objectionable.
License
$699 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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