IFI-B · Gluconapin Suppression Series

Specimen SS-F-BTR-314 // ZERO-BITTER · IFI-B

Gluconapin Supp · T5 Agrobacterium-B

Brassica oleracea

Broccoli/Kale

Tier VGluconapin Suppression Series

Target Trait

78%

% reduction

3-Butenyl glucosinolate reduction

Technical Specifications

SKU

SS-F-BTR-314

Genomic Edits

7

GC Content

47.5%

Construct Length

3,953 bp

Promoter System

35S CaMV / mas2

Expression System

T-DNA binary vector (pCAMBIA)

Host Strain

Agrobacterium tumefaciens EHA105

Receptor Target

TAS2R38 (suppression)

IFI Score

78

Pathway Edits

  • MAM3 RNAi reduces chain elongation toward C4 aliphatic glucosinolates
  • CYP79F1 RNAi suppresses Met-to-gluconapin C4 side-chain conversion
  • GSL-OH modification blocks 2-hydroxy derivative accumulation from gluconapin
  • AOP2 engineering redirects residual gluconapin flux away from bitter 2-hydroxy products

Key Interventions

MAM3 chain-elongation silencingCYP79F1 RNAiGSL-OH engineering

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
78
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier V Brassica oleracea lines with reduced gluconapin and progoitrin co-load, targeting breeders and food processors seeking consistent low-bitter profiles across the full C4 glucosinolate family.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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