Specimen SS-F-BTR-303 // ZERO-BITTER · IFI-B
Gluconapin Supp · T3 Cell-Free-A
Brassica oleracea
Broccoli/Kale
Target Trait
58%
% reduction
3-Butenyl glucosinolate reduction
Technical Specifications
SKU
SS-F-BTR-303
Genomic Edits
5
GC Content
43.8%
Construct Length
3,144 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
58
Pathway Edits
- MAM3 RNAi reduces chain elongation toward C4 aliphatic glucosinolates
- CYP79F1 RNAi suppresses Met-to-gluconapin C4 side-chain conversion
- GSL-OH modification blocks 2-hydroxy derivative accumulation from gluconapin
- AOP2 engineering redirects residual gluconapin flux away from bitter 2-hydroxy products
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier III Brassica oleracea lines with reduced gluconapin and progoitrin co-load, targeting breeders and food processors seeking consistent low-bitter profiles across the full C4 glucosinolate family.
License
$1499 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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