Specimen SS-F-BTR-272 // ZERO-BITTER · IFI-B
Sinigrin Redir · T4 Cell-Free-B
Brassica oleracea
Broccoli/Kale
Target Trait
65%
% reduction
Sinigrin bitter mass reduction
Technical Specifications
SKU
SS-F-BTR-272
Genomic Edits
6
GC Content
46.2%
Construct Length
3,628 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
65
Pathway Edits
- TGG1 myrosinase overexpression accelerates sinigrin hydrolysis kinetics in source tissue
- ESP RNAi eliminates epithiospecifier protein to redirect hydrolysis products toward AITC
- NSP1 RNAi removes nitrile-forming specifier competition, favoring the isothiocyanate channel
- BGLU enhanced expression improves glucosinolate turnover fidelity and completeness
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier IV Brassica lines with sinigrin redirected to AITC rather than accumulating as intact bitter GLS, suitable for cooked and fermented applications where glucosinolate hydrolysis is desirable but TAS2R38 activation is not.
License
$4049 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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