IFI-B · Sinigrin Redirection Series

Specimen SS-F-BTR-266 // ZERO-BITTER · IFI-B

Sinigrin Redir · T4 Agrobacterium-B

Brassica oleracea

Broccoli/Kale

Tier IVSinigrin Redirection Series

Target Trait

65%

% reduction

Sinigrin bitter mass reduction

Technical Specifications

SKU

SS-F-BTR-266

Genomic Edits

6

GC Content

46.7%

Construct Length

3,526 bp

Promoter System

35S CaMV / mas2

Expression System

T-DNA binary vector (pCAMBIA)

Host Strain

Agrobacterium tumefaciens EHA105

Receptor Target

TAS2R38 (suppression)

IFI Score

65

Pathway Edits

  • TGG1 myrosinase overexpression accelerates sinigrin hydrolysis kinetics in source tissue
  • ESP RNAi eliminates epithiospecifier protein to redirect hydrolysis products toward AITC
  • NSP1 RNAi removes nitrile-forming specifier competition, favoring the isothiocyanate channel
  • BGLU enhanced expression improves glucosinolate turnover fidelity and completeness

Key Interventions

TGG1 myrosinase enhancementESP RNAi silencingNSP1 suppression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
65
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV Brassica lines with sinigrin redirected to AITC rather than accumulating as intact bitter GLS, suitable for cooked and fermented applications where glucosinolate hydrolysis is desirable but TAS2R38 activation is not.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Zero-Bitter · IFI-BAll Modalities