IFI-B · Sinigrin Redirection Series

Specimen SS-F-BTR-261 // ZERO-BITTER · IFI-B

Sinigrin Redir · T3 Protoplast-A

Brassica oleracea

Broccoli/Kale

Tier IIISinigrin Redirection Series

Target Trait

55%

% reduction

Sinigrin bitter mass reduction

Technical Specifications

SKU

SS-F-BTR-261

Genomic Edits

5

GC Content

45.1%

Construct Length

3,230 bp

Promoter System

35S CaMV / RbcS

Expression System

PEG-mediated protoplast transfection

Host Strain

Isolated leaf mesophyll protoplasts

Receptor Target

TAS2R38 (suppression)

IFI Score

55

Pathway Edits

  • TGG1 myrosinase overexpression accelerates sinigrin hydrolysis kinetics in source tissue
  • ESP RNAi eliminates epithiospecifier protein to redirect hydrolysis products toward AITC
  • NSP1 RNAi removes nitrile-forming specifier competition, favoring the isothiocyanate channel
  • BGLU enhanced expression improves glucosinolate turnover fidelity and completeness

Key Interventions

TGG1 myrosinase enhancementESP RNAi silencingNSP1 suppression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
55
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier III Brassica lines with sinigrin redirected to AITC rather than accumulating as intact bitter GLS, suitable for cooked and fermented applications where glucosinolate hydrolysis is desirable but TAS2R38 activation is not.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Zero-Bitter · IFI-BAll Modalities