Sinigrin Redir · T2 Cell-Free-A

Brassica oleracea

Broccoli/Kale

Tier IISinigrin Redirection Series

Target Trait

45%

% reduction

Sinigrin bitter mass reduction

Technical Specifications

SKU

SS-F-BTR-255

Genomic Edits

GC Content

45.4%

Construct Length

2,917 bp

Promoter System

T7 / SP6 RNA polymerase

Expression System

Cell-free in vitro transcription/translation

Host Strain

In vitro wheat germ extract

Receptor Target

TAS2R38 (suppression)

IFI Score

Pathway Edits

TGG1 myrosinase overexpression accelerates sinigrin hydrolysis kinetics in source tissue
ESP RNAi eliminates epithiospecifier protein to redirect hydrolysis products toward AITC
NSP1 RNAi removes nitrile-forming specifier competition, favoring the isothiocyanate channel
BGLU enhanced expression improves glucosinolate turnover fidelity and completeness

Key Interventions

TGG1 myrosinase enhancementESP RNAi silencingNSP1 suppression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tier II Brassica lines with sinigrin redirected to AITC rather than accumulating as intact bitter GLS, suitable for cooked and fermented applications where glucosinolate hydrolysis is desirable but TAS2R38 activation is not.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Zero-Bitter · IFI-B All Modalities