Specimen SS-F-BTR-232 // ZERO-BITTER · IFI-B
Progoitrin Elim · T4 Cell-Free-B
Brassica oleracea
Broccoli/Kale
Target Trait
70%
% reduction
Progoitrin reduction
Technical Specifications
SKU
SS-F-BTR-232
Genomic Edits
7
GC Content
46.2%
Construct Length
3,928 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
70
Pathway Edits
- GSL-OH silencing (RNAi) blocks 2-hydroxylation of 3-butenyl glucosinolate
- AOP2 alternative splice-site engineering redirects sinigrin flux away from progoitrin
- AOP3 RNAi eliminates the competing 3-hydroxy glucosinolate branch
- GRL locus modification reduces rhamnosylation efficiency of aliphatic GLS
- FMO-GS-OX promoter attenuation lowers total aliphatic GLS throughput
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier IV broccoli and kale lines with clinically meaningful progoitrin reduction for fresh-market, minimally processed, and functional-food applications requiring validated TAS2R38 palatability improvement.
License
$4049 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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