IFI-B · Progoitrin Elimination Series

Specimen SS-F-BTR-225 // ZERO-BITTER · IFI-B

Progoitrin Elim · T4 Agrobacterium-A

Brassica oleracea

Broccoli/Kale

Tier IVProgoitrin Elimination Series

Target Trait

70%

% reduction

Progoitrin reduction

Technical Specifications

SKU

SS-F-BTR-225

Genomic Edits

7

GC Content

44.3%

Construct Length

4,009 bp

Promoter System

35S CaMV / mas2

Expression System

T-DNA binary vector (pCAMBIA)

Host Strain

Agrobacterium tumefaciens EHA105

Receptor Target

TAS2R38 (suppression)

IFI Score

70

Pathway Edits

  • GSL-OH silencing (RNAi) blocks 2-hydroxylation of 3-butenyl glucosinolate
  • AOP2 alternative splice-site engineering redirects sinigrin flux away from progoitrin
  • AOP3 RNAi eliminates the competing 3-hydroxy glucosinolate branch
  • GRL locus modification reduces rhamnosylation efficiency of aliphatic GLS
  • FMO-GS-OX promoter attenuation lowers total aliphatic GLS throughput

Key Interventions

GSL-OH RNAi knockdownAOP2 isoform switchAOP3 silencing

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
70
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tier IV broccoli and kale lines with clinically meaningful progoitrin reduction for fresh-market, minimally processed, and functional-food applications requiring validated TAS2R38 palatability improvement.

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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