Specimen SS-F-BTR-224 // ZERO-BITTER · IFI-B
Progoitrin Elim · T3 Cell-Free-B
Brassica oleracea
Broccoli/Kale
Target Trait
60%
% reduction
Progoitrin reduction
Technical Specifications
SKU
SS-F-BTR-224
Genomic Edits
6
GC Content
48.9%
Construct Length
3,581 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
60
Pathway Edits
- GSL-OH silencing (RNAi) blocks 2-hydroxylation of 3-butenyl glucosinolate
- AOP2 alternative splice-site engineering redirects sinigrin flux away from progoitrin
- AOP3 RNAi eliminates the competing 3-hydroxy glucosinolate branch
- GRL locus modification reduces rhamnosylation efficiency of aliphatic GLS
- FMO-GS-OX promoter attenuation lowers total aliphatic GLS throughput
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier III broccoli and kale lines with clinically meaningful progoitrin reduction for fresh-market, minimally processed, and functional-food applications requiring validated TAS2R38 palatability improvement.
License
$1549 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
Already purchased?