Specimen SS-F-BTR-213 // ZERO-BITTER · IFI-B
Progoitrin Elim · T2 Protoplast-A
Brassica oleracea
Broccoli/Kale
Target Trait
50%
% reduction
Progoitrin reduction
Technical Specifications
SKU
SS-F-BTR-213
Genomic Edits
4
GC Content
44.6%
Construct Length
2,803 bp
Promoter System
35S CaMV / RbcS
Expression System
PEG-mediated protoplast transfection
Host Strain
Isolated leaf mesophyll protoplasts
Receptor Target
TAS2R38 (suppression)
IFI Score
50
Pathway Edits
- GSL-OH silencing (RNAi) blocks 2-hydroxylation of 3-butenyl glucosinolate
- AOP2 alternative splice-site engineering redirects sinigrin flux away from progoitrin
- AOP3 RNAi eliminates the competing 3-hydroxy glucosinolate branch
- GRL locus modification reduces rhamnosylation efficiency of aliphatic GLS
- FMO-GS-OX promoter attenuation lowers total aliphatic GLS throughput
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier II broccoli and kale lines with clinically meaningful progoitrin reduction for fresh-market, minimally processed, and functional-food applications requiring validated TAS2R38 palatability improvement.
License
$699 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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