Specimen SS-F-BTR-208 // ZERO-BITTER · IFI-B
Progoitrin Elim · T1 Cell-Free-B
Brassica oleracea
Broccoli/Kale
Target Trait
40%
% reduction
Progoitrin reduction
Technical Specifications
SKU
SS-F-BTR-208
Genomic Edits
3
GC Content
46.0%
Construct Length
2,507 bp
Promoter System
T7 / SP6 RNA polymerase
Expression System
Cell-free in vitro transcription/translation
Host Strain
In vitro wheat germ extract
Receptor Target
TAS2R38 (suppression)
IFI Score
40
Pathway Edits
- GSL-OH silencing (RNAi) blocks 2-hydroxylation of 3-butenyl glucosinolate
- AOP2 alternative splice-site engineering redirects sinigrin flux away from progoitrin
- AOP3 RNAi eliminates the competing 3-hydroxy glucosinolate branch
- GRL locus modification reduces rhamnosylation efficiency of aliphatic GLS
- FMO-GS-OX promoter attenuation lowers total aliphatic GLS throughput
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier I broccoli and kale lines with clinically meaningful progoitrin reduction for fresh-market, minimally processed, and functional-food applications requiring validated TAS2R38 palatability improvement.
License
$349 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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