Polyphenol Concentration Scale V-PR-A

Vitis vinifera

Grape

Tier VPolyphenol Concentration Scale

Target Trait

6×

fold-change

Total polyphenol broad-spectrum accumulation index

Technical Specifications

SKU

SS-F-AST-997

Genomic Edits

GC Content

44.4%

Construct Length

5,484 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

600

Pathway Edits

MYB14 transcription factor overexpression for stilbene and flavonoid network co-activation
MYB15 transcription factor amplification for synergistic MYB14 polyphenol network induction
LAR downstream target activation (leucoanthocyanidin reductase) for PA branch output
ANR downstream target upregulation (anthocyanidin reductase) for epicatechin PA branch
TT12 vacuolar transport upregulation for broad-spectrum polyphenol vacuolar sequestration
TT19 glutathione S-transferase overexpression for PA and flavonoid sequestration efficiency
AHA10 H+-ATPase activation for vacuolar acidification and polyphenol stability
Protoplast PEG-fusion transient and stable integration protocol

Key Interventions

MYB14/MYB15 transcription factor cascade for broad-spectrum polyphenol network inductionLAR/ANR/TT12/TT19/AHA10 structural gene and transport co-activation for polyphenol accumulationCoordinated flavonoid and stilbene network amplification for maximum total polyphenol outputPEG-4000 mediated membrane fusion at 37°C

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

600

IFI-F

—

Use Case

Flagship ultra-high polyphenol concentrate production, industrial astringency ingredient manufacturing, premium functional food polyphenol extract production.

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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