IFI-A · Polyphenol Concentration Scale
Specimen SS-F-AST-983 // ASTRINGENCY · IFI-A
Polyphenol Concentration Scale III-IV-A
Vitis vinifera
Grape
Tier IIIPolyphenol Concentration Scale
Target Trait
4×
fold-change
Total polyphenol broad-spectrum accumulation index
Technical Specifications
SKU
SS-F-AST-983
Genomic Edits
8
GC Content
43.8%
Construct Length
4,244 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
400
Pathway Edits
- MYB14 transcription factor overexpression for stilbene and flavonoid network co-activation
- MYB15 transcription factor amplification for synergistic MYB14 polyphenol network induction
- LAR downstream target activation (leucoanthocyanidin reductase) for PA branch output
- ANR downstream target upregulation (anthocyanidin reductase) for epicatechin PA branch
- TT12 vacuolar transport upregulation for broad-spectrum polyphenol vacuolar sequestration
- TT19 glutathione S-transferase overexpression for PA and flavonoid sequestration efficiency
- AHA10 H+-ATPase activation for vacuolar acidification and polyphenol stability
- Cell-free wheat germ extract reconstitution system
Key Interventions
MYB14/MYB15 transcription factor cascade for broad-spectrum polyphenol network inductionLAR/ANR/TT12/TT19/AHA10 structural gene and transport co-activation for polyphenol accumulationCoordinated flavonoid and stilbene network amplification for maximum total polyphenol outputSP6/T7 dual-polymerase cell-free transcription/translation
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A400
IFI-F—
Use Case
Commercial total polyphenol concentrate production, mid-range astringency ingredient manufacturing, functional food R&D.
License
$1499 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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