IFI-A · Polyphenol Concentration Scale

Specimen SS-F-AST-978 // ASTRINGENCY · IFI-A

Polyphenol Concentration Scale III-AG-B

Vitis vinifera

Grape

Tier IIIPolyphenol Concentration Scale

Target Trait

4.6×

fold-change

Total polyphenol broad-spectrum accumulation index

Technical Specifications

SKU

SS-F-AST-978

Genomic Edits

9

GC Content

48.2%

Construct Length

4,739 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

460

Pathway Edits

  • MYB14 transcription factor overexpression for stilbene and flavonoid network co-activation
  • MYB15 transcription factor amplification for synergistic MYB14 polyphenol network induction
  • LAR downstream target activation (leucoanthocyanidin reductase) for PA branch output
  • ANR downstream target upregulation (anthocyanidin reductase) for epicatechin PA branch
  • TT12 vacuolar transport upregulation for broad-spectrum polyphenol vacuolar sequestration
  • TT19 glutathione S-transferase overexpression for PA and flavonoid sequestration efficiency
  • AHA10 H+-ATPase activation for vacuolar acidification and polyphenol stability
  • T-DNA right-border optimized integration cassette
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

MYB14/MYB15 transcription factor cascade for broad-spectrum polyphenol network inductionLAR/ANR/TT12/TT19/AHA10 structural gene and transport co-activation for polyphenol accumulationCoordinated flavonoid and stilbene network amplification for maximum total polyphenol outputAgrobacterium co-cultivation with acetosyringone inductionVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
460
IFI-F
—

Use Case

Commercial total polyphenol concentrate production, mid-range astringency ingredient manufacturing, functional food R&D. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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