Specimen SS-F-AST-968 // ASTRINGENCY · IFI-A
Polyphenol Concentration Scale I-IV-B
Vitis vinifera
Grape
Target Trait
2.3×
fold-change
Total polyphenol broad-spectrum accumulation index
Technical Specifications
SKU
SS-F-AST-968
Genomic Edits
6
GC Content
46.0%
Construct Length
3,567 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
230
Pathway Edits
- MYB14 transcription factor overexpression for stilbene and flavonoid network co-activation
- MYB15 transcription factor amplification for synergistic MYB14 polyphenol network induction
- LAR downstream target activation (leucoanthocyanidin reductase) for PA branch output
- ANR downstream target upregulation (anthocyanidin reductase) for epicatechin PA branch
- TT12 vacuolar transport upregulation for broad-spectrum polyphenol vacuolar sequestration
- TT19 glutathione S-transferase overexpression for PA and flavonoid sequestration efficiency
- AHA10 H+-ATPase activation for vacuolar acidification and polyphenol stability
- Cell-free wheat germ extract reconstitution system
- Enhanced flux via 5'UTR synthetic leader sequence optimization
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Total polyphenol accumulation research, entry-level broad-spectrum astringency engineering, academic MYB transcription factor network studies. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.
License
$349 · Tier I License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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