Sorghum Condensed Tannin Reduction IV-IV-B

Sorghum bicolor

Sorghum

Tier IVSorghum Condensed Tannin Reduction

Target Trait

74.75%

% reduction

Condensed tannin anti-nutritional reduction (% reduction from baseline)

Technical Specifications

SKU

SS-F-AST-952

Genomic Edits

GC Content

46.2%

Construct Length

3,968 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

74.8

Pathway Edits

SG4-RNAi silencing (sorghum flavan-4-ol synthase RNAi) for phlobaphene/PA precursor suppression
ANR-RNAi knockdown (anthocyanidin reductase RNAi) for epicatechin extension unit depletion
TT19-RNAi suppression (vacuolar transport RNAi) for PA sequestration efficiency reduction
Cell-free wheat germ extract reconstitution system
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

SG4-RNAi suppression of sorghum flavan-4-ol synthase for phlobaphene PA reductionANR-RNAi depletion of epicatechin extension unit supply for condensed tannin reductionTT19-RNAi vacuolar transport knockdown for PA accumulation limitationSP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

74.8

IFI-F

—

Use Case

Industrial sorghum tannin reduction at scale, premium food-grade sorghum ingredient production, anti-nutritional factor mitigation. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Astringency · IFI-A All Modalities