IFI-A · Sorghum Condensed Tannin Reduction

Specimen SS-F-AST-936 // ASTRINGENCY · IFI-A

Sorghum Condensed Tannin Reduction II-IV-B

Sorghum bicolor

Sorghum

Tier IISorghum Condensed Tannin Reduction

Target Trait

51.75%

% reduction

Condensed tannin anti-nutritional reduction (% reduction from baseline)

Technical Specifications

SKU

SS-F-AST-936

Genomic Edits

5

GC Content

47.3%

Construct Length

3,274 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

51.8

Pathway Edits

  • SG4-RNAi silencing (sorghum flavan-4-ol synthase RNAi) for phlobaphene/PA precursor suppression
  • ANR-RNAi knockdown (anthocyanidin reductase RNAi) for epicatechin extension unit depletion
  • TT19-RNAi suppression (vacuolar transport RNAi) for PA sequestration efficiency reduction
  • Cell-free wheat germ extract reconstitution system
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

SG4-RNAi suppression of sorghum flavan-4-ol synthase for phlobaphene PA reductionANR-RNAi depletion of epicatechin extension unit supply for condensed tannin reductionTT19-RNAi vacuolar transport knockdown for PA accumulation limitationSP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
51.8
IFI-F
—

Use Case

Craft low-tannin sorghum food ingredient development, specialty grain palatability improvement. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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