IFI-A · Sorghum Condensed Tannin Reduction
Specimen SS-F-AST-935 // ASTRINGENCY · IFI-A
Sorghum Condensed Tannin Reduction II-IV-A
Sorghum bicolor
Sorghum
Tier IISorghum Condensed Tannin Reduction
Target Trait
45%
% reduction
Condensed tannin anti-nutritional reduction (% reduction from baseline)
Technical Specifications
SKU
SS-F-AST-935
Genomic Edits
4
GC Content
45.4%
Construct Length
2,877 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
45
Pathway Edits
- SG4-RNAi silencing (sorghum flavan-4-ol synthase RNAi) for phlobaphene/PA precursor suppression
- ANR-RNAi knockdown (anthocyanidin reductase RNAi) for epicatechin extension unit depletion
- TT19-RNAi suppression (vacuolar transport RNAi) for PA sequestration efficiency reduction
- Cell-free wheat germ extract reconstitution system
Key Interventions
SG4-RNAi suppression of sorghum flavan-4-ol synthase for phlobaphene PA reductionANR-RNAi depletion of epicatechin extension unit supply for condensed tannin reductionTT19-RNAi vacuolar transport knockdown for PA accumulation limitationSP6/T7 dual-polymerase cell-free transcription/translation
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A45
IFI-F—
Use Case
Craft low-tannin sorghum food ingredient development, specialty grain palatability improvement.
License
$699 · Tier II License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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