IFI-A · Smooth Astringency Grade

Specimen SS-F-AST-918 // ASTRINGENCY · IFI-A

Smooth Astringency Grade V-PR-B

Vitis vinifera

Grape

Tier VSmooth Astringency Grade

Target Trait

3.68×

fold-change

Low-molecular-weight proanthocyanidin smooth mouthfeel index

Technical Specifications

SKU

SS-F-AST-918

Genomic Edits

8

GC Content

46.9%

Construct Length

4,401 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

Polyphenol-astringency axis

IFI Score

368

Pathway Edits

  • LAR-short overexpression (truncated leucoanthocyanidin reductase variant) for low-DP catechin unit production
  • ANR-low expression (low-activity anthocyanidin reductase) to limit PA chain elongation
  • TT12-alt alternative vacuolar transporter preferentially sequesters short-chain PAs for smooth-finish accumulation
  • Protoplast PEG-fusion transient and stable integration protocol
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

LAR-short truncation for low-DP catechin unit supply limiting proanthocyanidin chain elongationANR-low activity attenuation for reduced epicatechin extension and short PA distributionTT12-alt selective vacuolar transport for smooth-finish low-MW proanthocyanidin accumulationPEG-4000 mediated membrane fusion at 37°CVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
368
IFI-F
—

Use Case

Flagship smooth-finish tannin ingredient production, premium velvety mouthfeel ingredient manufacturing, next-generation smooth astringency beverage formulation. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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