Smooth Astringency Grade V-BL-B

Vitis vinifera

Grape

Tier VSmooth Astringency Grade

Target Trait

3.68×

fold-change

Low-molecular-weight proanthocyanidin smooth mouthfeel index

Technical Specifications

SKU

SS-F-AST-916

Genomic Edits

GC Content

48.7%

Construct Length

4,367 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

Polyphenol-astringency axis

IFI Score

368

Pathway Edits

LAR-short overexpression (truncated leucoanthocyanidin reductase variant) for low-DP catechin unit production
ANR-low expression (low-activity anthocyanidin reductase) to limit PA chain elongation
TT12-alt alternative vacuolar transporter preferentially sequesters short-chain PAs for smooth-finish accumulation
Biolistic tungsten-particle coating for leaf/seed explant delivery
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

LAR-short truncation for low-DP catechin unit supply limiting proanthocyanidin chain elongationANR-low activity attenuation for reduced epicatechin extension and short PA distributionTT12-alt selective vacuolar transport for smooth-finish low-MW proanthocyanidin accumulationHigh-pressure helium particle acceleration at 1100 psiVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

368

IFI-F

—

Use Case

Flagship smooth-finish tannin ingredient production, premium velvety mouthfeel ingredient manufacturing, next-generation smooth astringency beverage formulation. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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