Smooth Astringency Grade V-AG-A

Vitis vinifera

Grape

Tier VSmooth Astringency Grade

Target Trait

3.2×

fold-change

Low-molecular-weight proanthocyanidin smooth mouthfeel index

Technical Specifications

SKU

SS-F-AST-913

Genomic Edits

GC Content

45.9%

Construct Length

3,936 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

320

Pathway Edits

LAR-short overexpression (truncated leucoanthocyanidin reductase variant) for low-DP catechin unit production
ANR-low expression (low-activity anthocyanidin reductase) to limit PA chain elongation
TT12-alt alternative vacuolar transporter preferentially sequesters short-chain PAs for smooth-finish accumulation
T-DNA right-border optimized integration cassette

Key Interventions

LAR-short truncation for low-DP catechin unit supply limiting proanthocyanidin chain elongationANR-low activity attenuation for reduced epicatechin extension and short PA distributionTT12-alt selective vacuolar transport for smooth-finish low-MW proanthocyanidin accumulationAgrobacterium co-cultivation with acetosyringone induction

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

320

IFI-F

—

Use Case

Flagship smooth-finish tannin ingredient production, premium velvety mouthfeel ingredient manufacturing, next-generation smooth astringency beverage formulation.

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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