IFI-A · Smooth Astringency Grade

Specimen SS-F-AST-912 // ASTRINGENCY · IFI-A

Smooth Astringency Grade IV-IV-B

Vitis vinifera

Grape

Tier IVSmooth Astringency Grade

Target Trait

3.14×

fold-change

Low-molecular-weight proanthocyanidin smooth mouthfeel index

Technical Specifications

SKU

SS-F-AST-912

Genomic Edits

7

GC Content

46.2%

Construct Length

3,888 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

314

Pathway Edits

  • LAR-short overexpression (truncated leucoanthocyanidin reductase variant) for low-DP catechin unit production
  • ANR-low expression (low-activity anthocyanidin reductase) to limit PA chain elongation
  • TT12-alt alternative vacuolar transporter preferentially sequesters short-chain PAs for smooth-finish accumulation
  • Cell-free wheat germ extract reconstitution system
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

LAR-short truncation for low-DP catechin unit supply limiting proanthocyanidin chain elongationANR-low activity attenuation for reduced epicatechin extension and short PA distributionTT12-alt selective vacuolar transport for smooth-finish low-MW proanthocyanidin accumulationSP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
314
IFI-F
—

Use Case

Industrial smooth-finish tannin ingredient production, premium velvety mouthfeel ingredient formulation. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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