IFI-A · Smooth Astringency Grade

Specimen SS-F-AST-882 // ASTRINGENCY · IFI-A

Smooth Astringency Grade I-AG-B

Vitis vinifera

Grape

Tier ISmooth Astringency Grade

Target Trait

1.49×

fold-change

Low-molecular-weight proanthocyanidin smooth mouthfeel index

Technical Specifications

SKU

SS-F-AST-882

Genomic Edits

4

GC Content

45.8%

Construct Length

2,745 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

Polyphenol-astringency axis

IFI Score

149

Pathway Edits

  • LAR-short overexpression (truncated leucoanthocyanidin reductase variant) for low-DP catechin unit production
  • ANR-low expression (low-activity anthocyanidin reductase) to limit PA chain elongation
  • TT12-alt alternative vacuolar transporter preferentially sequesters short-chain PAs for smooth-finish accumulation
  • T-DNA right-border optimized integration cassette
  • Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

LAR-short truncation for low-DP catechin unit supply limiting proanthocyanidin chain elongationANR-low activity attenuation for reduced epicatechin extension and short PA distributionTT12-alt selective vacuolar transport for smooth-finish low-MW proanthocyanidin accumulationAgrobacterium co-cultivation with acetosyringone inductionVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
149
IFI-F
—

Use Case

Smooth tannin research, entry-level low-MW PA engineering, academic smooth mouthfeel mechanism studies. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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