Tea-Black Fermentation V-IV-B

Camellia sinensis

Tea

Tier VTea-Black Fermentation

Target Trait

4.14×

fold-change

Theaflavin/thearubigin equivalent oxidative coupling index

Technical Specifications

SKU

SS-F-AST-880

Genomic Edits

GC Content

47.4%

Construct Length

4,735 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

Polyphenol-astringency axis

IFI Score

414

Pathway Edits

PPO1 overexpression (polyphenol oxidase 1) for catechin ortho-quinone formation and theaflavin initiation
LAC3 laccase amplification for complementary oxidative coupling of flavan-3-ol substrates
CYP78A5 upregulation for subsidiary oxidative modification of flavan-3-ol substrates
peroxidase-1 co-expression for H2O2-dependent oxidative coupling in thearubigin formation
Cell-free wheat germ extract reconstitution system
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

PPO1-driven catechin ortho-quinone formation for theaflavin benzotropolone ring condensationLAC3/peroxidase-1 complementary oxidative coupling for thearubigin analog formationCYP78A5 auxiliary oxidative modification for expanded oxidative astringency architectureSP6/T7 dual-polymerase cell-free transcription/translationVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

414

IFI-F

—

Use Case

Flagship black tea analog ingredient production, ultra-premium theaflavin standardization, pharmaceutical-grade oxidative coupling polyphenol manufacturing. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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