Tea-Black Fermentation IV-BL-B

Camellia sinensis

Tea

Tier IVTea-Black Fermentation

Target Trait

3.51×

fold-change

Theaflavin/thearubigin equivalent oxidative coupling index

Technical Specifications

SKU

SS-F-AST-868

Genomic Edits

GC Content

47.0%

Construct Length

4,320 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

Polyphenol-astringency axis

IFI Score

351

Pathway Edits

PPO1 overexpression (polyphenol oxidase 1) for catechin ortho-quinone formation and theaflavin initiation
LAC3 laccase amplification for complementary oxidative coupling of flavan-3-ol substrates
CYP78A5 upregulation for subsidiary oxidative modification of flavan-3-ol substrates
peroxidase-1 co-expression for H2O2-dependent oxidative coupling in thearubigin formation
Biolistic tungsten-particle coating for leaf/seed explant delivery
Enhanced flux via 5'UTR synthetic leader sequence optimization

Key Interventions

PPO1-driven catechin ortho-quinone formation for theaflavin benzotropolone ring condensationLAC3/peroxidase-1 complementary oxidative coupling for thearubigin analog formationCYP78A5 auxiliary oxidative modification for expanded oxidative astringency architectureHigh-pressure helium particle acceleration at 1100 psiVariant B enhanced: iterative codon optimization for host-matched expression

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

351

IFI-F

—

Use Case

Industrial black tea analog ingredient production, premium theaflavin manufacturing, oxidative coupling polyphenol scale-up. Enhanced variant for premium or research-grade applications requiring peak astringency fidelity.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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