IFI-A · Tea-Black Fermentation
Specimen SS-F-AST-863 // ASTRINGENCY · IFI-A
Tea-Black Fermentation III-IV-A
Camellia sinensis
Tea
Tier IIITea-Black Fermentation
Target Trait
2.5×
fold-change
Theaflavin/thearubigin equivalent oxidative coupling index
Technical Specifications
SKU
SS-F-AST-863
Genomic Edits
6
GC Content
43.8%
Construct Length
3,644 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
Polyphenol-astringency axis
IFI Score
250
Pathway Edits
- PPO1 overexpression (polyphenol oxidase 1) for catechin ortho-quinone formation and theaflavin initiation
- LAC3 laccase amplification for complementary oxidative coupling of flavan-3-ol substrates
- CYP78A5 upregulation for subsidiary oxidative modification of flavan-3-ol substrates
- peroxidase-1 co-expression for H2O2-dependent oxidative coupling in thearubigin formation
- Cell-free wheat germ extract reconstitution system
Key Interventions
PPO1-driven catechin ortho-quinone formation for theaflavin benzotropolone ring condensationLAC3/peroxidase-1 complementary oxidative coupling for thearubigin analog formationCYP78A5 auxiliary oxidative modification for expanded oxidative astringency architectureSP6/T7 dual-polymerase cell-free transcription/translation
IFI Subscale Profile
IFI Profile
IFI-S—
IFI-U—
IFI-B—
IFI-K—
IFI-C—
IFI-P—
IFI-A250
IFI-F—
Use Case
Commercial black tea analog ingredient production, mid-range oxidative coupling R&D, beverage black tea formulation.
License
$1499 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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